Modifications to the hand-held Gene Gun: improvements for in vitro Biolistic transfection of organotypic neuronal tissue
نویسندگان
چکیده
منابع مشابه
Biolistic Transfection of Organotypic Brain Slices and Dissociated Cells
The introduction of genetic material into cells and tissues of interest remains a rate-limiting step for molecular investigations in many fields, especially neurobiology. Many different methods have been developed to transfect cells—DNA-mediated gene transfer, microinjection, electroporation, lipofection, and viral transfection—and for many cell types, gene transfer using these methods has beco...
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Transfection of DNA has been invaluable for biological sciences and with recent advances to organotypic brain slice preparations, the effect of various heterologous genes could thus be investigated easily while maintaining many aspects of in vivo biology. There has been increasing interest to transfect terminally differentiated neurons for which conventional transfection methods have been fraug...
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Biolistic transfection is a physical means of transfecting cells by bombarding tissue with high velocity DNA coated particles. We provide a detailed protocol for biolistic transfection of rat hippocampal slices, from the initial preparation of DNA coated bullets to the final shooting of the organotypic slice cultures using a gene gun. Gene gun transfection is an efficient and easy means of tran...
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Our aim is the biolistic transfection of organotypic cultures of rat visual cortex with plasmids coding for neurotrophic factors, which then become expressed for limited periods of time during postnatal ontogenesis. Out of two commercially available devices, we adopted the handheld 'Helios Gene Gun' instead of the stationary PDS-1000He (both Biorad, Munich, Germany). This device allows multiple...
متن کاملNano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles
BACKGROUND Biolistic transfection is proving an increasingly popular method of incorporating DNA or RNA into cells that are difficult to transfect using traditional methods. The technique routinely uses 'microparticles', which are ~1 μm diameter projectiles, fired into tissues using pressurised gas. These microparticles are efficient at delivering DNA into cells, but cannot efficiently transfec...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2001
ISSN: 0165-0270
DOI: 10.1016/s0165-0270(01)00457-5